Composite

Part:BBa_K3781215:Design

Designed by: Nicolas Bayer   Group: iGEM21_TU_Kaiserslautern   (2021-10-14)


L1_sAP_Strep_mCerulean_GST, MocloMania Composite


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1492
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1492
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 6
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1492
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1492
    Illegal NgoMIV site found at 823
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1464
    Illegal SapI.rc site found at 1536


Design Notes

This composite part was assembled from four basic parts that are part of the MocloMania collection. Due to the highly standardizes set of overhangs that all our basic parts comply with, assembly could be achieved by a simple Moclo ligation. Assembly of this L1 part allowed us to verify the correct construction of its sub-parts. Furthermore, it was built to test expression, secretion and purification of recombinant mCerulean and examine thefunctional fluorescence of the resulting protein.


Source

For information on the origin of the genetic sequences present in this composite part, please consult the individual basic part pages.